Genetic diversity analysis and phylogenetic reconstruction of groupers Epinephelus spp. from Madura Island, Indonesia based on partial sequence of CO1 gene

Abstract. Basith A, Abinawanto, Kusrini E, Yasman. 2021. Genetic diversity analysis and phylogenetic reconstruction of groupers Epinephelus spp. from Madura Island, Indonesia based on partial sequence CO1 gene. Biodiversitas 22: 4282-4290. Groupers populations in Indonesia, particularly East Java are indicated to be over-fished, thereby requiring data collection more accurate genetic resources as an important step for grouper conservation. A total 14 samples the Epinepheplus were obtained fish landing port Island. The 617 bp gene was utilized tree reconstruction. is value haplotype (Hd) nucleotide (?). Reconstruction includes neighbor-joining (NJ) implementing K2P substitution model, while maximum likelihood (ML) conducted by HKY+G+I both which evaluated employing a bootstrap 1000 replications. Analysis distance between species that farthest E. heniochus fasciatus 0.189, closest erythrurus ongus 0.099. Intrapopulation high with details Hd=0.978 ?=0.12107. Furthermore, NJ ML demonstrated similar topology observed Island grouped into 7 clades, coioides, bleekeri, areolatus, erythrurus, heniochus, fasciatus, ongus.